Pesticide compositions for control of fungal disease in cultivated crops

ABSTRACT

The present invention provides an effective method for controlling foliage and root fungus infection in cultivated crops. A fungicide composition in the form of an aqueous formulation which is applied to pre-harvest crops contains ingredients such as etridiazole, potassium bicarbonate, sodium carbonate, potassium oleate, potassium caprylate and xanthan gum. The combination of potassium oleate, potassium caprylate and xanthan gum functions as an effective spreader-sticker medium for forming an adherent coating on plant foliage surfaces, and for saturating ground soil. The coating exhibits both immediate and long duration fungicidal activities.

BACKGROUND OF THE INVENTION

The control of phytopathogenic fungi is of great economic importance since fungal growth on plants or on parts of plants inhibits production of foliage, fruit or seed, and the overall quality of a cultivated crop. About 25 percent of all fungal diseases in agriculture and horticulture are caused by powdery mildew phytopathogens.

Because of the vast economic ramifications of fungal propagation in agricultural and horticultural cultivations, a broad spectrum of fungicidal and fungistatic products have been developed for general and specific applications.

Of particular interest with respect to the present invention embodiments are fungicide compositions which contain an inorganic bicarbonate and an optional carbonate compound. It is known that bicarbonate and carbonate compounds exhibit fungicidal properties for agricultural purposes.

Phytopathology, 48, 169 (1931) by R. H. Marloth describes studies involving the physiology of fungi. The reference reports studies which demonstrate that sodium and potassium bicarbonate and carbonate salts are toxic to fungi such as Penicillium italicum and Penicillium digitatum.

U.S. Pat. No. 1,560,558 discloses the use of salts such as lithium carbonate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, potassium carbonate and ammonium bicarbonate as fungicide ingredients.

U.S. Pat. No. 4,599,233 describes a fungicide composition which consists of sodium bicarbonate in combination with a surface active food emulsifier such as sorbitan monostearate.

Japanese patent 53118523 describes the combination of sodium bicarbonate and lecithin as an active agent for the control of agricultural and fruit storage fungus diseases.

Japanese patent 56043207 describes a biocidal composition containing sodium bicarbonate and a polyglycerol fatty acid ester. The biocide controls Penicillium digitatum on oranges, Sphaerotheca fuligenea on cucumbers, Piricularia oryzae on rice, and mosaic virus on tomatoes.

Japanese patent 60097909 describes a soil fungicide prepared by admixing slaked lime with sodium bicarbonate, potassium bicarbonate, boric acid and phenolphthalein.

German patent DE 2927994 describes a fungicide which consists of sodium bicarbonate incorporated into a food-compatible surfactant such as saccharose laurate.

Japanese patent 57062208 describes horticultural fungicides in which the addition of sodium bicarbonate to polyoxin or thiophanatemethyl increases the fungicidal activity of the organic biocide against Botrytis cinerea on cucumbers.

Japanese patent 58023609 describes an agricultural fungicide composed of a mixture of sodium bicarbonate or potassium bicarbonate with cupric hydroxide, basic copper carbonate or basic copper sulfate. The combination of ingredients exhibits a synergistic fungicidal effect against cucumber early blight, tomato wilt, rice sheath blight, rice blast and citrus canker.

There remains a continuing need for improved methods for providing preventive and curative fungicidal activity for the protection of cultivated plants with a minimum of phytotoxic side effects.

Accordingly, it is an object of this invention to provide a bicarbonate salt-containing fungicide composition for controlling fungal diseases in cultivated plants.

It is another object of this invention to provide a method for controlling foliage and soil-borne fungal diseases in agricultural and horticultural crops with an aqueous fungicide formulation having a synergistic combination of inorganic and organic fungicidal-active ingredients.

Other objects and advantages of the present invention shall become apparent from the accompanying description and examples.

DESCRIPTION OF THE INVENTION

One or more objects of the present invention are accomplished by the provision of a method for controlling fungal disease in cultivated plants which comprises contacting the plant matter with a fungicidally effective application of an aqueous formulation which has a content comprising (1) between about 0.002-2 weight percent of an ingredient selected from the group consisting of alkali metal and ammonium bicarbonates; (2) between about 0-1 weight percent of a basic ingredient selected from the group consisting of alkali metal and ammonium carbonates; (3) between about 0.001-2 weight percent of etridiazole; (4) between about 0.05-1 weight percent of a surfactant ingredient selected from the group consisting of alkali metal and ammonium C₁₂ -C₃₀ aliphatic-containing carboxylate, sulfonate, sulfate and phosphate salts; (5) between about 0-0.25 weight percent of a hydrotrope ingredient selected from the group consisting of alkali metal and ammonium C₇ -C₁₀ fatty acid salts; and (6) between about 0-0.5 weight percent of a hydrophilic polymer ingredient; based on the formulation weight.

In another embodiment this invention provides a fungicide composition which is a dry blend formulation comprising (1) between about 0.02-20 parts by weight of an ingredient selected from the group consisting of alkali metal and ammonium bicarbonates; (2) between about 0-10 parts by weight of an ingredient selected from the group consisting of alkali metal and ammonium carbonates; (3) between about 0.01-20 parts by weight of etridiazole; (4) between about 0.5-10 parts by weight of a surfactant ingredient selected from the group consisting of alkali metal and ammonium C₁₂ -C₃₀ aliphatic-containing carboxylate, sulfonate, sulfate and phosphate salts; (5) between about 0-2.5 parts by weight of a hydrotrope ingredient selected from the group consisting of alkali metal and ammonium C₇ -C₁₀ fatty acid salts; and (6) between about 0-5 parts by weight of a hydrophilic polymer ingredient.

The organic fungicide ingredient is a commercial product which corresponds to the structural formula: ##STR1## The compound is chemically identified as etridiazole, which is sold under tradenames such as TERRAZOLE (Uniroyal Chemical Company) and TRUBAN (Grace Sierra). The formal nomenclature is 5-ethoxy-3-trichloromethyl-1,2,4-thiadiazole.

The application of a present invention pesticide formulation to agricultural and horticultural plant-growth sites to combat fungi also is effective for eradication of insects such as aphids, mites, and the like.

An invention aqueous formulation can be prepared by pre-blending the solid ingredients, and then dispersing the blended admixture in an aqueous medium to a selected concentration of bicarbonate and etridiazole ingredients. Optionally, the ingredients can be individually dispersed in the aqueous medium.

An invention aqueous formulation can be prepared as a concentrated medium which is diluted further before usage, or the dilute aqueous formulation can be prepared directly by adding the individual ingredients or a pre-blend of ingredients to an aqueous medium.

An invention dilute aqueous fungicidal solution is in a ready-to-use form which can be applied directly to the foliage of plants, bushes and trees, such as by electrodynamic spraying techniques. An adherent film-like coating forms on the plant matter surfaces after the aqueous medium has evaporated. An invention formulation has particular advantages for controlling soil-borne fungi.

The bicarbonate salt ingredient of an invention fungicide formulation is sodium bicarbonate, potassium bicarbonate or ammonium bicarbonate, or any mixture thereof.

Illustrative of a bicarbonate salt ingredient in a formulation are sodium, potassium or ammonium bicarbonate; or mixtures such as sodium bicarbonate and potassium bicarbonate; sodium bicarbonate and ammonium bicarbonate; potassium bicarbonate and ammonium bicarbonate; sodium bicarbonate, potassium bicarbonate and ammonium bicarbonate; and the like. Multiple bicarbonate salts are fungicidally more effective than a single bicarbonate salt, as demonstrated in Example V.

The basic carbonate salt ingredient of an invention dilute aqueous fungicide formulation typically is between about 0.01-1 weight percent of sodium carbonate, potassium carbonate, ammonium carbonate or lithium carbonate, or any mixture thereof.

Multiple inorganic salt compounds can be utilized in a broad range of molar quantities relative to each other. In one aspect the molar quantity of a carbonate salt compound is determined by pH control considerations when aqueous formulations are prepared. The content of a carbonate salt compound can be varied to control the pH of an aqueous fungicide formulation at a desired level in the range of about 7.5-11. An aqueous fungicide formulation of the present invention tends to have a higher fungicidal activity at higher pH values.

The inclusion of a carbonate salt ingredient in an aqueous fungicide formulation is an important feature of the present invention means of controlling fungal disease in agricultural and horticultural plant life and plant soil. In addition to providing alkaline pH control, the carbonate salt potentiates the fungicidal activity of the bicarbonate salt ingredient. Further, under ambient atmospheric conditions, the carbonate salt in the adherent film-like coating on plant matter and soil particle surfaces reacts with carbon dioxide and moisture to form bicarbonate salt in situ over a prolonged duration. An invention aqueous fungicide formulation exhibits both immediate fungicidal activity after application, and long duration fungicidal activity under ambient conditions.

The surfactant ingredient of an invention fungicide formulation is an anionic surface active derivative selected from alkali metal and ammonium C₁₂ -C₃₀ aliphatic-containing carboxylate, sulfonate, sulfate and phosphate salts. The C₁₂ -C₃₀ aliphatic content of an anionic surfactant can be in a single aliphatic radical such as sodium oleate, or in multiple aliphatic radicals such as sodium ditridecylsulfosuccinate. These anionic surfactants provide superior dispersant activity when an invention aqueous formulation is prepared.

An invention aqueous formulation is in the form of a stable aqueous dispersion which exhibits excellent spreader-sticker and film-forming properties when applied to plant foliage and soil.

Surfactants which provide suitable dispersant properties are anionic compounds such as alkyl sulfates and alkyl ether sulfates having 12-20 carbon atoms in the alkyl group. The ether sulfates can contain about 1-10 oxyethylene groups in the molecule. The sulfate surfactants can be in the form of sodium, potassium, ammonium, lower alkylamine or lower alkanolamine salts. Amine compounds are illustrated by dimethylamine, trimethylamine, diethanolamine, triethanolamine, and the like.

Other suitable anionic surfactants include sodium and potassium alkylbenzenesulfonates, in which the alkyl group contains about 12-18 carbon atoms; sodium alkyl glycerol ether sulfonates; sodium coconut oil fatty acid monoglyceride sulfates and sulfonates; sodium salts of sulfuric acid esters of oxyethylated fatty alcohols; sodium sulfosuccinate esters; condensation products of fatty acids with sarcosine; monoester or diester phosphates of C₁₂ -C₁₈ fatty alcohols in the form of sodium, potassium or ammonium salts (e.g., Gafac PE-510, GAF Corporation); and the like. Anionic surfactants are described in references such as U.S. Pat. No. 4,528,039 and U.S. Pat. No. 5,037,818, incorporated herein by reference.

Other suitable anionic surfactants are C₁₂ -C₂₂ fatty acid salts selected from alkali metal and ammonium salts of natural straight chain and synthetic branched chain fatty acids, which have a saturated or unsaturated structure. Illustrative of natural fatty acids are myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, arachidic acid, behenic acid, cetoleic acid, and the like.

A C₁₂ -C₂₂ fatty acid salt ingredient can consist of two or more saturated or unsaturated carboxylic acids such as those derived from beef and mutton tallow, lard, cottonseed oil, palm oil, and the like.

A preferred aspect of the present invention is the usage of the surfactant ingredient in combination with between about 0.01-0.25 weight percent of a hydrotropic ingredient in an invention aqueous fungicide formulation. The term "hydrotrope" as employed herein refers to a straight-chain or branched-chain C₇ -C₁₀ fatty acid salt which functions to stabilize and maintain the solubility of the surfactant ingredient in the aqueous medium, as the concentration of the bicarbonate salt-containing aqueous medium increases by water evaporation after application of the aqueous fungicide formulation to plant foliage. If the anionic surfactant is salted out of the aqueous solution, it is ineffective for spreading the bicarbonate salt and etridiazole ingredients for maximum coverage of fungus-infected plant foliage. The combination of the surfactant and hydrotrope ingredients promote the biocidal efficacy of the bicarbonate and etridiazole ingredients.

Suitable C₇ -C₁₀ fatty acid hydrotrope compounds include sodium, potassium, ammonium, lower alkylamine and lower alkanolamine salts of enanthylic acid, caprylic acid, pelargonic acid, capric acid, and the like, or any mixture thereof. Hydrotrope usage is described in publications such as U.S. Pat. No. 4,444,573; incorporated by reference.

The optional hydrophilic polymer ingredient of an invention fungicide formulation is selected from water-soluble organic polymers which exhibit pseudoplastic rheological properties in an aqueous medium. A preferred content of hydrophilic polymer in a dilute aqueous fungicide formulation is between about 0.01-0.5 weight percent. A preferred content of hydrophilic polymer in a dry blend formulation is between about 0.1-5 parts by weight.

The term "water-soluble" as employed herein refers to a hydrophilic polymer ingredient which has a solubility of at least about one gram per 100 grams of water at 25° C.

The term "pseudoplastic" as employed herein refers to the rheological behavior of an aqueous solution containing a dissolved hydrophilic polymer ingredient, in which the apparent viscosity of the aqueous solution decreases with increasing shear rate.

Illustrative of water-soluble polymers which exhibit pseudoplastic properties in an aqueous medium are gum arabic, gum karaya, gum tragacanth, guar gum, locust bean gum, xanthan gum, carrageenan, alginate salt, casein, dextran, pectin, agar, 2-hydroxyethyl starch, 2-aminoethyl starch, 2-hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose salt, cellulose sulfate salt, polyacrylamide, alkali metal salts of the maleic anhydride copolymers, alkali metal salts of poly(meth)acrylate, and the like.

Many of the water-soluble polymers are large volume commercial products. Sodium carboxymethyl cellulose (CMC) is available in powder or granular form having a particle size of 50-200 microns. CMC is available in a degree of substitution (DS) range of 0.38-1.4.

One type of preferred aqueous formulation in accordance with the present invention is one containing two or more alkali metal or ammonium bicarbonates, alkali metal or ammonium carbonate, alkali metal or ammonium C₁₂ -C₂₂ fatty acid salt, alkali metal or ammonium C₇ -C₁₀ fatty acid salt, a hydrocolloid gum, and etridiazole.

The ingredients in an invention fungicide formulation can be selected to include nitrogen, phosphorus and potassium elements in a fertilizer-effective ratio and quantity that qualifies the composition to function as a fertilizer in addition to its function as a fungicide, when applied to cultivated crops. When an aqueous solution containing fertilizer elements is sprayed on plant foliage, there is direct absorption of the fertilizer elements into the leaves. A typical fertilizer ratio of NPK is 5-10-5.

Besides nitrogen, phosphorus and potassium, an invention fungicide fertilizer formulation can contain trace elements, and other essential elements as exemplified by sulfur as contained in a compound such as water-soluble sodium bisulfite or thiourea.

An invention fungicide formulation can include one or more other biologically active ingredients, such as those which exhibit herbicidal, insecticidal or plant growth regulating activity.

A fungicide formulation of the present invention has a novel combination of properties for the practice of pesticide control in agricultural and horticultural applications.

The bicarbonate and carbonate ingredients exhibit fungicidal properties, and the efficacy of the etridiazole ingredient is enhanced by the presence of bicarbonate and carbonate salts. A lesser quantity of organic pesticide ingredient can be employed to achieve a desired degree of pest control, as demonstrated in Example I of the present specification.

A present invention aqueous fungicide medium can be formulated to exhibit no phytotoxicity, or to minimize the toxic effects of salt stress on plants by the bicarbonate and carbonate ingredients.

A present invention fungicide formulation provides particular advantage for the control of infectious phytopathogenic fungi which thrive under acidic soil conditions, such as Rhizoctonia, Pythium, Sclerotinea, Phytophthora and Fusarium species.

A significant feature of a preferred invention fungicide formulation is the presence of surfactant, hydrotrope and hydrophilic polymer ingredients, which function as a spreader-sticker medium when the fungicide formulation is applied to plant foliage as an aqueous solution. An applied aqueous solution forms an adherent coating of ingredients on plant foliage or fruit, and saturates the rooted soil. The surfactant ingredient aids in spreading and sticking the fungicide formulation ingredients to the foliage or fruit to which it is applied. The hydrotrope ingredient maintains the surface activity of the surfactant under application conditions. The hydrophilic polymer ingredient increases the amount of aqueous fungicide composition which adheres to the plant matter surfaces because of its static high apparent viscosity. During a spraying procedure, the hydrophilic polymer ingredient contributes a low mobile viscosity to the spray solution, which facilitates the spraying action. After spraying, the resultant solid coating resists drifting under wind conditions, and exhibits humectant properties in addition to enhanced fungicidal activity.

Another important advantage of an invention fungicide formulation derives from the water-solubility of contained inorganic salts and spreader-sticker ingredients. The film-like coating of an invention fungicide formulation on plant matter surfaces can be removed readily by water-washing. Conventional fungicide formulations which contain a petroleum-based or fatty acid ester type spreader-sticker ingredient leave an oily residue on treated plant foliage or fruit which is difficult to remove.

A present invention fungicide formulation is adapted for application to field crops to prevent or eradicate fungal disease, or to inhibit re-infection by phytopathogenic fungi. Typically the aqueous formation is sprayed on a field crop at least twice during the growth cycle, with a time interval between about 5-20 days.

A present invention fungicide formulation has particular advantage for the control of fungal diseases such as Sclerotinea homeocarpa in crops such as ornamental stock, bedding plants, turf, cereals, vegetables, fruits, cotton, and the like.

Illustrative of particular crops are pre-harvest and post-harvest stages of grape, apple, cucumber, potato, rose, peanut, and the like.

A present invention fungicide formulation can be biocidally effective against Diplocarpin rosae, Didymella bryoniae, Colletotrichum orbiculare, Botrytis cinerea, Penicillium digitatum, Guignardia bidwellii and Puccinia hordei.

A present invention fungicide formulation also can be biocidally effective against powdery mildew species which include Erysiphe asperifolium, Erysiphe betae, Erysiphe cichoracearum, Erysiphe cruciferarum, Erysiphe galeopsidis, Erysiphe graminis, Erysiphe pisi, Erysiphe polygoni, Erysiphe ranunculi, Erysiphe trifolii, Erysiphe verbasci, Microsphaera alphitoides, Oidium euonymi-japonicae, Oidium hortensiae, Podosphaera leucotricha, Podosphaera tridactyla, Sphaerotheca alchemillae, Sphaerotheca fuliginea, Sphaerotheca maculans, Sphaerotheca pannosa, Uncinula bicornis and Uncinula necator.

A present invention fungicide formulation also can be biocidally effective against downy mildew species which include Peronospora antirrhini, Peronospora arborescens, Peronospora destructor, Peronospora farinosa, Peronospora ficariae, Peronospora galligena, Peronospora grisea, Peronospora lamii, Peronospora parasitica, Peronospora sparsa, Peronospora trifoliorum, Peronospora viciae, Pseudoperonospora humuli, and Plasmopara vitacola.

The following examples are further illustrative of the present invention. The components and specific ingredients are presented as being typical, and various modifications can be derived in view of the foregoing disclosure within the scope of the invention.

EXAMPLE I

This Example illustrates the synergistic fungicidal activity exhibited by a mixture of a bicarbonate salt and a commercial organic fungicide against soil-borne fungus growth.

The fungicide test ingredients are mixed with cornmeal agar medium at 55° C. Twenty milliliter aliquots of the medium are distributed among petri dishes. Plugs of Pythium sp. fungus, previously grown on cornmeal agar medium, individually are set in the respective test media. The resulting fungal colony size is measured after a four day period. Differences in fungal growth are attributable to the inhibitory effects of the active fungicide test ingredients present in the nutritive medium. Four replications of each test mixture are made.

A.

The following data tabulation is from a first comparative study. The data demonstrate a synergy between bicarbonate salt and TERRAZOLE (etridiazole; Uniroyal Chemical Company) in inhibiting the growth rate of Pythium sp.

    ______________________________________                                                              Growth Rate Std                                           Test Medium          (cm/day)    Dev.                                          ______________________________________                                         Water                        2.55      0.09                                    NaHCO.sub.3     0.03   M     0.63      0.08                                    NaHCO.sub.3     0.06   M     0.00      0.00                                    KHCO.sub.3      0.03   M     1.03      0.13                                    KHCO.sub.3      0.06         0.73      0.08                                    KHCO.sub.3      0.09   M     0.35      0.05                                    Etr 3.2 × 10.sup.-3 ul/ml (A)                                                                         1.33      0.33                                    Etr 2.0 × 10.sup.-4 ul/ml (B)                                                                         2.35      0.05                                    A + NaHCO.sub.3 0.03   M     0.04      0.04                                    B + KHCO.sub.3  0.03   M     0.80      0.00                                    B + KHCO.sub.3  0.06   M     0.45      0.05                                    B + KHCO.sub.3  0.09   M     0.08      0.02                                    ______________________________________                                    

The data show that both sodium bicarbonate and potassium bicarbonate provide synergistic inhibition of growth rate when mixed with sub-lethal concentrations of etridiazole. Similar results are obtained with formulations when tested for inhibition of Sclerotinea Homeocarpa fungus.

B.

The following data tabulation is from a second comparative study, which is conducted in the same manner as the first study.

    ______________________________________                                                              Growth Rate                                                                               Std                                            Test Medium          (cm/day)   Dev.                                           ______________________________________                                         Water                    2.13       0.00                                       NaHCO.sub.3    0.005 M   1.93       0.03                                       NaHCO.sub.3    0.01 M    1.74       0.08                                       NaHCO.sub.3    0.03 M    0.75       0.05                                       KHCO.sub.3     0.005 M   2.09       0.01                                       KHCO.sub.3     0.01 M    1.98       0.02                                       KHCO.sub.3     0.03 M    1.07       0.04                                       NH.sub.4 HCO.sub.3                                                                            0.005 M   2.13       0.00                                       NH.sub.4 HCO.sub.3                                                                            0.01 M    0.98       0.10                                       NH.sub.4 HCO.sub.3                                                                            0.03 M    0.00       0.00                                       Etr. 5.55 × 10.sup.-3 ul/ml (A)                                                                   0.44       0.01                                       A + NaHCO.sub.3                                                                               0.005 M   0.24       0.01                                       A + NaHCO.sub.3                                                                               0.01 M    0.11       0.11                                       A + NaHCO.sub.3                                                                               0.03 M    0.00       0.00                                       A + KHCO.sub.3 0.005 M   0.20       0.03                                       A + KHCO.sub.3 0.01 M    0.13       0.03                                       A + KHCO.sub.3 0.03 M    0.00       0.00                                       A + NH.sub.4 HCO.sub.3                                                                        0.005 M   0.23       0.05                                       A + NH.sub.4 HCO.sub.3                                                                        0.01 M    0.21       0.03                                       A + NH.sub.4 HCO.sub.3                                                                        0.03 M    0.00       0.00                                       ______________________________________                                    

The results confirm the synergistic fungal inhibitory activity of all three bicarbonate salts with etridiazole. The advantages of using combinations of bicarbonates with etridiazole include:

1. the ability to obtain higher levels of fungicidal activity at lower levels of either fungicide lessens the chance of phytotoxic effects;

2. the different modes of action of each fungicide lessens the likelihood of a fungus becoming immune to the combination; and

3. the use of a lower level of fungicide results in less undesirable residues being released in the environment.

EXAMPLE II

This Example illustrates the synergistic fungicidal activity exhibited by a mixture of a bicarbonate salt and a commercial organic fungicide against fungal infection in turf.

Bend grass (Agrostis palustris) is inoculated with a fungus culture for test purposes. Greenhouse 3.5 inch pots of bend grass are grown to a height of 2.5 cm, and then inoculated with a rye grain culture of Phythium sp. Inoculation consists of 6-10 rye grains placed in the middle of each spot of soil level, and the plants are misted for several hours after inoculation.

The treatments are applied 24 hours after inoculation, and data is collected 7 and 14 days after treatments. Ten pots are employed for each treatment.

A 25% emulsifiable concentrate of TRUBAN Concentrate (Grace Sierra) is diluted with water to a concentration of 0.6 g of etridiazole per liter (×conc.). The Invention Formulation powder is diluted with water to a concentration of 5.9 g per liter (×conc.). All treatments are applied at 40 ml per pot.

The Invention Formulation powder is a blend of the following ingredients:

    ______________________________________                                                             wt. %                                                      ______________________________________                                         potassium bicarbonate                                                                              37                                                         potassium carbonate 14                                                         urea                34                                                         sodium dioctyl sulfosuccinate                                                                      9                                                          sodium caprylate    6                                                          ______________________________________                                    

The effectiveness of the treatments is scored as follows:

0=no mildew, 1=10% coverage, 2=30% coverage,

3=50% coverage, 4=70% coverage,

5=90% coverage.

The following aqueous formulations are compared:

    ______________________________________                                         Treatments        Average  Std. Dev.                                           ______________________________________                                         uninoculated      0        0                                                   inoculated        4.1      0.7                                                 1 × TRUBAN  1.5      0.5                                                 0.5 × TRUBAN and                                                         0.5 × Inv. Form.                                                                           0.7      0.6                                                 0.25 × TRUBAN and                                                        0.5 × Inv. Form.                                                                           1.2      0.6                                                 0.25 × TRUBAN and                                                        0.25 × Inv. Form.                                                                          1.3      0.9                                                 0.25 Inv. Form.   2.3      0.6                                                 ______________________________________                                    

The comparative data demonstrate a synergistic fungicidal activity between the TRUBAN ingredient and the Invention Formulation potassium bicarbonate ingredient for control of Pythium sp. infection of bend grass turf.

EXAMPLE III

This Example illustrates the preparation of a concentrated aqueous fungicide formulation.

    ______________________________________                                                           Parts                                                        ______________________________________                                         etridiazole         4.00                                                       potassium bicarbonate                                                                              20.00                                                      potassium oleate    15.00                                                      potassium caprylate 5.00                                                       xanthan gum         2.00                                                       butylated hydroxytoluene                                                                           0.02                                                       (BHT)                                                                          water               57.98                                                      ______________________________________                                    

The aqueous concentrate is prepared by dissolving the potassium bicarbonate in the water, and then with stirring adding the other ingredients.

The prepared concentrated aqueous formulation is diluted 1 part to 40 parts of water. The diluted formulation is effective for prevention of foliage and root fungal diseases on cultivated plants. The formulation inhibits propagation of Sphaerotheca fuligenea powdery mildew and Colletotrichum orbiculare anthracnose on cucurbits.

EXAMPLE IV

This Example illustrates the preparation of a fungicide formulation in accordance with the present invention.

    ______________________________________                                                           Parts                                                        ______________________________________                                         etridiazole         20                                                         potassium bicarbonate                                                                              50                                                         sodium carbonate    5                                                          potassiuze palmitate                                                                               15                                                         sodium caprate      10                                                         sodium carboxymethyl-                                                                              5                                                          cellulose.sup.(1)                                                              water               50                                                         ______________________________________                                          .sup.(1) Aldrich Chemical Co., CMC of 3000-6000 centipoises, intrinsic         viscosity of 2% aqueous solution at 25° C.                        

The solid ingredients ar blended, and the blend is suspended in water to form an aqueous emulsion.

The emulsion formulation is diluted with water to 0.2% by weight of bicarbonate ingredient. The diluted formulation is effective as a fungicide medium with turf infected with Erysiphe spp., Colletotrichum spp. and/or Sclerotinea homeocarpa.

EXAMPLE V

This Example illustrates the preparation of a fungicide powder concentrate in accordance with the present invention.

A blend of the following ingredients is prepared:

    ______________________________________                                                           Parts                                                        ______________________________________                                         etridiazole         10                                                         sodium bicarbonate  40                                                         potassium bicarbonate                                                                              20                                                         potassium carbonate 5                                                          sodium oleate       25                                                         sodium caprylate    8                                                          ______________________________________                                    

The formulated concentrated powder is diluted with water by the dispersion of 1 part of the powder blend into 100 parts of water. The resulting solution is sprayed onto a field crop of peanut plants.

The formulation is effective for controlling Rhizoctonia, Pythium, Phytophthora and Fusarium infections of the crop and rooted soil. The aqueous formulation is applied twice to the crop and soil, with a 14 day interval.

The multiple bicarbonate salts are fungicidally more effective than an equivalent quantity of a single bicarbonate salt for control of the fungal diseases.

The blend is reformulated with the addition of four parts by weight of guar gum to enhance the spreader-sticker properties of the formulation for control of fungus infections. 

What is claimed is:
 1. A method for controlling fungal disease in cultivated plants which comprises contacting the plant matter with a fungicidally effective application of an aqueous formulation which has a content comprising (1) between about 0.002-2 weight percent of an ingredient selected from the group consisting of alkali metal and ammonium bicarbonates; (2) between about 0-1 weight percent of a basic ingredient selected from the group consisting of alkali metal and ammonium carbonates; (3) between about 0.001-2 weight percent of etridiazole; (4) between about 0-1 weight percent of a surfactant ingredient; (5) between about 0-0.25 weight percent of a hydrotrope ingredient selected from the group consisting of alkali metal and ammonium C₇ -C₁₀ fatty acid salts; and (6) between about 0-0.5 weight percent of a hydrophilic polymer ingredient; based on the formulation weight.
 2. A method in accordance with claim 1 wherein the bicarbonate ingredient is sodium bicarbonate, potassium bicarbonate or ammonium bicarbonate, or any mixture thereof.
 3. A method in accordance with claim 1 wherein the basic ingredient is between about 0.01-1 weight percent of sodium carbonate, potassium carbonate, ammonium carbonate or lithium carbonate, or any mixture thereof, and the pH of the formulation is in the range between about 7.5-11.
 4. A method in accordance with claim 1 wherein the surfactant ingredient is between about 0.05-1 weight percent of a member selected from the group consisting of alkali metal and ammonium C₁₂ -C₃₀ aliphatic-containing carboxylate, sulfonate, sulfate and phosphate salts.
 5. A method in accordance with claim 1 wherein the surfactant ingredient is palmitic acid, stearic acid, oleic acid or palm fatty acid distillate salt or any mixture thereof.
 6. A method in accordance with claim 1 wherein the surfactant ingredient is benzenesulfonate or sulfosuccinate salt or any mixture thereof.
 7. A method in accordance with claim 1 wherein the surfactant ingredient is an oxyalkylene-containing sulfate salt.
 8. A method in accordance with claim 1 wherein the hydrotrope ingredient is between about 0.01-0.25 weight percent of enanthylic acid, caprylic acid, pelargonic acid or capric acid salt or any mixture thereof.
 9. A method in accordance with claim 1 wherein the hydrophilic polymer ingredient comprises between about 0.01-0.5 weight percent of a hydrocolloid gum.
 10. A method in accordance with claim 1 wherein the hydrophilic polymer ingredient comprises between about 0.01-0.5 weight percent of a cellulosic derivative.
 11. A method in accordance with claim 1 wherein the hydrophilic polymer ingredient comprises between about 0.01-0.5 weight percent of a starch derivative.
 12. A method in accordance with claim 1 wherein the hydrophilic polymer ingredient comprises between about 0.01-0.5 weight percent of xanthan gum or guar gum.
 13. A method in accordance with claim 1 wherein the cultivated plants are in a growth cycle and the aqueous formulation is applied to the plants at least twice with a time interval between about 5-20 days.
 14. A method in accordance with claim 1 wherein the cultivated plants are one of a crop selected from the group consisting of peanut, cotton, vegetables, turf, and ornamentals.
 15. A method in accordance with claim 1 wherein the disease being controlled is a soil-borne fungus.
 16. A method in accordance with claim 1 wherein the disease being controlled is a Rhizoctonia, Pythium, Sclerotinea, Phytophthora or Fusarium fungus.
 17. A method in accordance with claim 1 wherein the disease being controlled is a powdery mildew or downy mildew fungus.
 18. A method in accordance with claim 1 wherein the disease being controlled is soil-borne Pythium sp. or Sclerotinea Homeocarpa fungus.
 19. A fungicide composition which is a dry blend formulation comprising (1) between about 0.02-20 parts by weight of an ingredient selected from the group consisting of alkali metal and ammonium bicarbonates; (2) between about 0-10 parts by weight of an ingredient selected from the group consisting of alkali metal and ammonium carbonates; (3) between about 0.01-20 parts by weight of etridiazole; (4) between about 0-10 parts by weight of a surfactant ingredient; (5) between about 0-2.5 parts by weight of a hydrotrope ingredient selected from the group consisting of alkali metal and ammonium C₇ -C₁₀ fatty acid salts; and (6) between about 0-5 parts by weight of a hydrophilic polymer ingredient.
 20. A fungicide composition in accordance with claim 19 wherein the surfactant ingredient is between about 0.5-10 parts by weight of a member selected from the group consisting of alkali metal and ammonium C₁₂ -C₃₀ aliphatic-containing carboxylate, sulfonate, sulfate and phosphate salts.
 21. A fungicide composition in accordance with claim 19 which has a fertilizer effective ratio and quantity of nitrogen, phosphorus and potassium elements.
 22. A fungicide composition in accordance with claim 19 which is a water-diluted formulation for fungicidally effective application to cultivated crops for control of foliar-borne and soil-borne fungal disease. 